Eps8 blocks elongation of activin treated animal caps. (A,B) Control and Eps8-injected animal cap explants remain rounded in the absence of activin. (C,D) Control explants elongate extensively in the presence of activin, whereas Eps8 expression inhibits elongation. (E) RT-PCR analysis shows that Eps8 expression does not block activin-mediated induction of the mesodermal markers Xbra and MyoD. ODC is a control for mRNA isolation, reverse transcription, and gel loading.
Roffers-Agarwal et al. BMC Cell Biology 2005 6:36 doi:10.1186/1471-2121-6-36