Additional file 1.


Time series of GFP-HPS3 and acidic vesicle live cell imaging. GFP-HPS3 expressing HPS3 null fibroblast was imaged at 37°C following a 2 h incubation at 20°C. Time series (from inset in Figure 2a[A] and Figure 2b) showing a transient association of GFP-HPS3 with Lysotracker Red stained vesicles as they exit the perinuclear area.

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Helip-Wooley et al. BMC Cell Biology 2005 6:33   doi:10.1186/1471-2121-6-33