Surface expression of APP is increased by dyn I K44A overexpression. A. HEK-M3 cells were transiently transfected with APP695 and dyn I K44A or empty vector. After 48 hours, cells were surface biotinylated, lysed, and incubated with streptavidin-agarose beads. Biotinylated proteins were immunoblotted with antibodies to the APP C-terminal. Full-length, biotinylated, endogenous and co-transfected APP are indicated by arrows. B. HEK-695 cells were transiently transfected with dyn I K44A or empty vector. Immunoblot analysis of cell lysates showed increased formation of the APP C-terminal fragment (C83) generated by α-secretase cleavage in cells transfected with the dynamin mutant. C. Results from B were quantitated, normalized and expressed as means ± SEM from 3 experiments. *, p < 0.05 by paired t-test.
Carey et al. BMC Cell Biology 2005 6:30 doi:10.1186/1471-2121-6-30