Actinomycin D treatment alters the co-localization of Tim50a with snRNPs. HeLa cells transfected with GFP-tagged Tim50a (green) were untreated or subject to actinomycin D, followed by fixation and staining with the anti-Sm antibody Y12 (red). The overlay of the images is shown in the last column. For untreated cells, arrowheads mark the location of Cajal bodies and arrows show speckles. For actinomycin D treated cells, arrows mark accumulations of Tim50a while arrowheads show enlarged Sm speckles.
Xu et al. BMC Cell Biology 2005 6:29 doi:10.1186/1471-2121-6-29