Localization of the SMC complex in live cells depends on several physiological factors. A) Localization of ScpA-YFP in wild-type cells (arrowheads indicate central SMC foci in a newborn cell, two central foci in a slightly older cell, and bipolar foci in a yet older cell), and B) SMC-YFP in wild type cells. C) defective localization of the Walker A motif SMC-YFP mutant protein, D) localization of ScpA-YFP in a strain expressing the C-motif SMC mutant allele only, E) localization of ScpA-YFP, 1 hour after addition of nalidixic acid to block gyrase activity (arrowheads indicate remaining foci). F) localization of Spo0J-GFP, 1 hour after addition of nalidixic acid. G-J) ScpA-YFP in a dnaB ts strain, G) 20–30 min, H) 30–45 min or I) 60 min after shift to 42°C, J) 60 min after shift back from 42°C to 25°C. Arrowheads indicate two bipolar and one central ScpA-YFP foci in (G). White lines indicate septa between cells. Grey bars 2 μm.
Mascarenhas et al. BMC Cell Biology 2005 6:28 doi:10.1186/1471-2121-6-28