Syntaxin 2 and 3 can be transported to their intracellular sites along actin filaments. The NRK cells were incubated in the presence of 1 mM NEM for 15 minutes and then further incubated for one or two hours. Syntaxin 2 and 3 were visualized using syntaxin 2 and 3 anti-sera and LRSC-conjugated goat anti-rabbit IgG; actin filaments were stained with Oregon Green phalloidin. Enlargements of the boxed areas are shown below. The arrows show the colocalization of syntaxin 2 and 3 staining with actin staining in filament-like structures after one hour of NEM-treatment. Conventional fluorescence images were viewed using an Olympus AX70 fluorescence microscope. Bars, 10 μm.
Band and Kuismanen BMC Cell Biology 2005 6:26 doi:10.1186/1471-2121-6-26