The intracellular syntaxin 2 or 3 antibody labelled structures do not represent newly synthesized syntaxin 2 or 3 proteins. The NRK cells were treated with 50 μg/ml cycloheximide for two hours. The cells were fixed with 0.08 M lysine-0.01 M periodate-2% paraformaldehyde and permeabilized with 0.05% saponin. Syntaxin 2 and 3 were visualized using syntaxin 2 and 3 anti-sera and LRSC-conjugated goat anti-rabbit IgG. Conventional fluorescence images were viewed using an Olympus AX70 fluorescence microscope. Bars,10 μm.
Band and Kuismanen BMC Cell Biology 2005 6:26 doi:10.1186/1471-2121-6-26