Table 1

Summary of average lifetimes for GFP emission under various conditions

Transfection

Fig

Treatment

Area analysed

Avg Lifetime (ns)


GFP-PKC

2

None

cytoplasm

2.2

None

nucleus

2.2

3

TPA

cytoplasm

2.2

TPA

nucleus

2.3

GFP-PKC/DsRed-cav

4

None

cytoplasm

2.4

None

cytoplasm

2.5

5

Ca2+

cytoplasm

1.6*

Ca2+

nucleus

2.0

6

TPA

cytoplasm excluding peripheral

1.5*

TPA

nucleus

2.0

TPA

peripheral

1.8*

7

Bradykinin

cytoplasm

1.6*


*reduced lifetime indicating PKC-GFP-DsRed-cav interaction

Stubbs et al. BMC Cell Biology 2005 6:22   doi:10.1186/1471-2121-6-22

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