Lifetime imaging of GFP-PKC co-expressed with DsRed-cav in CHO cells: effect of bradykinin. 2P-FLIM images were collected as described in the legend to Figure 2. Cells were treated with 5 nM bradykinin for 3 min before mounting and fixation as described in Methods. The area in the cytoplasm outlined in red was analysed as shown in the colour coded inset (τ avg: 1.6 ns), showing PKC interaction with caveolin.
Stubbs et al. BMC Cell Biology 2005 6:22 doi:10.1186/1471-2121-6-22