Velocity distributions. Histograms show changes in the velocity distribution profiles in response to surface coatings for the different extracellular matrices laminin, collagen IV and collagen I (all at 10 ug/ml coating concentration) in comparison to mock-coated plastic for KM101 cells (A) and MG-63 cells (B). Velocity measurements on individual objects were filtered according to criteria for single cells as described (see Methods) and were compiled over an elapsed time from 24 to 48 hours from three experiments. The horizontal axes indicate velocity measurement bins from which the number of objects included within each bin are determined as shown on the vertical axis (faint horizontal lines represent 1000 objects). The different colours within each bar indicate cell track length (from bottom to top: grey indicates tracks of 11 or more segments, violet: 10 segments, light-blue: 9 segments, bright-blue: 8, green: 7, black: 6, yellow: 5, dark-blue: 4, and red: 3 segments).
Bahnson et al. BMC Cell Biology 2005 6:19 doi:10.1186/1471-2121-6-19