Ratio of area-based cell counts to manual cell counts. Area-based cell counts were automatically estimated by dividing the total area of segmented objects in each image by the average area of a single cell for each cell type, KM101 (red squares) and MG-63 cells (green circles). Manual counts were scored using a custom program that marks cells when clicked on, and increments a total count. A total of 90 images were analysed for each cell type across a range of confluence levels. The individual counts were grouped and averaged according to manual count levels; maximum values corresponded to 100% confluence for each cell type. Trend lines were based upon an exponential fit.
Bahnson et al. BMC Cell Biology 2005 6:19 doi:10.1186/1471-2121-6-19