3D visualization of matched cell tracks. Cell positions from the first manual tracking exercise (vertical blue markers) that matched either the second manual tracking exercise (horizontal yellow markers) or the automated tracking (red globes) were plotted in 3D with x and y coordinates in the plane of the paper and with increasing elapsed time (scan = 30 minutes) represented by the z axis coming "up" toward the viewer (similar to Figure 2). Lines connect markers by unique Track ID numbers, so that detailed features of track continuity may be examined. For clarity, only cell positions from one example culture well are shown (mock-coated plastic).
Bahnson et al. BMC Cell Biology 2005 6:19 doi:10.1186/1471-2121-6-19