Correlation between manual and automated velocity measurement. Velocity from the automated method (Panel A) or from the second manual replicate trial (Panel B) were plotted in reference to corresponding matched velocities from the first manual tracking exercise (y axis = dependent variable, x axis = independent variable). Increasing frequency of points is indicated by density contour lines and red shaded intensity (color scale). The dashed line indicates the line of identity and the solid line indicates the regression line for each comparison. Regression statistics were as follows: For automated tracking (y) versus the first manual tracking (x) (Panel A), the slope and intercept were 0.79 ± 0.02 and 0.03 ± 0.01, respectively with Pearson's correlation, r, equal to 0.81 ± 0.01. For the second (y) versus the first (x) manual tracking (Panel B), the slope and intercept were 0.84 ± 0.02 and 0.07 ± 0.01, respectively with Pearson's correlation equal to 0.86 ± 0.01.
Bahnson et al. BMC Cell Biology 2005 6:19 doi:10.1186/1471-2121-6-19