Example tracks from KM101 cells on different surface coatings. Single time-point images shown were acquired at 36 hours elapsed time from KM101 cells seeded into a 384 well plate pre-treated with 10 ug/ml solutions of (A) laminin, (B) collagen I, (C) collagen IV, (D) and mock coated. Tracks (green lines) have been superimposed on the images to indicate the paths of cells or clusters of cells over the previous set of 15 images (7.5 hours). Track continuity is broken in cases where two objects collide, and tracks are reinitiated upon object separation. Longer tracks reflect uninterrupted tracks and higher cell velocities. Within a track, each straight-line segment represents the distance between object centroid positions in successive images taken 30 minutes apart.
Bahnson et al. BMC Cell Biology 2005 6:19 doi:10.1186/1471-2121-6-19