Additional File 2.
Transient expression of ECFP-CB(SC)-EYFP in LCLC-103H cells (fluorescence channels, phase contrast, and superimposition of the last fluorescence frames). The double-tagged construct is expressed in the cytoplasm and accumulated within the nucleus. No significant differences among the two fluorescence channels can be observed suggesting that the polypeptide remains intact and is imported into the cell nucleus as a whole. Only the expressing cells undergo cell death and reveal membrane blebbing, which is typical for apoptotic processes. WFM; obj. Neofluar® 10×/0.30 Ph. Δt = 3 min, total time = 250 min. Video format: MPEG1. The abbreviation "MM" (mature message) is used equivalently to the term "SC" (single chain).
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Bestvater et al. BMC Cell Biology 2005 6:16 doi:10.1186/1471-2121-6-16