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Open Access Research article

The C-terminal subunit of artificially truncated human cathepsin B mediates its nuclear targeting and contributes to cell viability

Felix Bestvater, Claudia Dallner and Eberhard Spiess*

Author Affiliations

Deutsches Krebsforschungszentrum, PO Box 101949, D-69009 Heidelberg, Germany

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BMC Cell Biology 2005, 6:16  doi:10.1186/1471-2121-6-16

Published: 4 April 2005

Additional files

Additional File 1:

Transient expression of CB(SC)-EYFP in LCLC-103H cells (phase contrast, fluorescence channel, and inverse grey value representation of the fluorescence signal). The sequence describes the formation of granules, their fusion, and the transport to the nucleus accompanied by breakup of the Golgi. WFM, LD Apochromat® 40×/0.60 Ph. Δt = 5 min, total time = 195 min. Video format: MPEG1. The abbreviation "MM" (mature message) is used equivalently to the term "SC" (single chain).

Format: MPG Size: 2.3MB Download file

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Open Data

Additional File 2:

Transient expression of ECFP-CB(SC)-EYFP in LCLC-103H cells (fluorescence channels, phase contrast, and superimposition of the last fluorescence frames). The double-tagged construct is expressed in the cytoplasm and accumulated within the nucleus. No significant differences among the two fluorescence channels can be observed suggesting that the polypeptide remains intact and is imported into the cell nucleus as a whole. Only the expressing cells undergo cell death and reveal membrane blebbing, which is typical for apoptotic processes. WFM; obj. Neofluar® 10×/0.30 Ph. Δt = 3 min, total time = 250 min. Video format: MPEG1. The abbreviation "MM" (mature message) is used equivalently to the term "SC" (single chain).

Format: MPG Size: 3.3MB Download file

Playing the movie within this page requires QuickTime 6.4 or later and JavaScript. Read more

Open Data