Table 2 |
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|
Rate of basal and cAMP-induced Mn2+-influx. Amoebae were challenged with 1 or 100 μM Mn2+ either with or without 1 μM cAMP. Cells were preincubated with 0.1 mM EGTA as outlined in Methods. Mn2+ quenching of fura-2-dextran fluorescence was tested in H5-buffer and is expressed as decrease in fluorescence units/sec (mean ± s.e.m.). Numbers in brackets give number of cells tested and number of experiments. |
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|
Stimulation |
Preincubation |
|
|
none |
EGTA (0.1 mM) |
|
|
|
||
|
1 μM Mn2+ |
0 (121/5) |
0.37 ± 0.2 (142/3) |
|
1 μM Mn2+/1 μM cAMP |
0 (135/5) |
1.4 ± 0.1 (207/4) |
|
100 μM Mn2+ |
0 (109/3) |
|
|
100 μM Mn2+/1 μM cAMP |
1.0 ± 0.2 (292/9) |
|
|
|
||
|
Schaloske et al. BMC Cell Biology 2005 6:13 doi:10.1186/1471-2121-6-13 |
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