Table 2

Rate of basal and cAMP-induced Mn2+-influx. Amoebae were challenged with 1 or 100 μM Mn2+ either with or without 1 μM cAMP. Cells were preincubated with 0.1 mM EGTA as outlined in Methods. Mn2+ quenching of fura-2-dextran fluorescence was tested in H5-buffer and is expressed as decrease in fluorescence units/sec (mean ± s.e.m.). Numbers in brackets give number of cells tested and number of experiments.

Stimulation

Preincubation

none

EGTA (0.1 mM)


1 μM Mn2+

0 (121/5)

0.37 ± 0.2 (142/3)

1 μM Mn2+/1 μM cAMP

0 (135/5)

1.4 ± 0.1 (207/4)

100 μM Mn2+

0 (109/3)

100 μM Mn2+/1 μM cAMP

1.0 ± 0.2 (292/9)


Schaloske et al. BMC Cell Biology 2005 6:13   doi:10.1186/1471-2121-6-13

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