Table 1

Determination of Ca2+-fluxes in partially purified storage compartments of the iplA- mutant and of wild type. Ca2+-sequestering vesicles were prepared as outlined in Methods. Measurements were performed with the pellet and supernatant fraction. ATP-induced uptake and release activated by different agents is given as nmol Ca2+-uptake/min and mg of protein and pmol Ca2+-release/tube, respectively (mean ± s.d.). In release experiments 60–75 μl of pellet and 120–140 μl of supernatant fraction were used per tube. Numbers in brackets give number of experiments; n.d.: not determined.

iplA-

Wt

Stimulation

Fraction

Fraction

Pellet

Supernatant

Pellet

Supernatant


Uptake (nmol/min*mg)

1 mM ATP

1.96 ± 0.55 (4)

0.28 ± 0.15 (4)

1.87 ± 0.74 (3)

0.38 ± 0.16 (3)

Release (pmol/tube)

10 μM AA

360 ± 227 (8)

761 ± 218 (6)

396 ± 122 (6)

961 ± 374 (2)

40 μM Thapsigargin

570 ± 250 (6)

170 ± 73 (6)

582 ± 123 (5)

265 ± 135 (2)

6 μM XestosponginC

201 ± 55 (4)

n.d.

276 ± 46 (3)

n.d.

2 μM Ionomycin

447 ± 147 (5)

147 ± 41 (4)

580± 173 (3)

193 ± 76 (2)


Schaloske et al. BMC Cell Biology 2005 6:13   doi:10.1186/1471-2121-6-13

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