Fatty acids activate Ca2+-fluxes in iplA- amoebae. (A) 60 μM AA evoked a transient decrease in [Ca2+]e representing Ca2+-influx; measurement was done at t6 (B) After preincubating amoebae with the SERCA-type Ca2+-ATPase blocker BHQ (100 μM) for 20 min to inhibit uptake of Ca2+ into internal storage compartments, the AA-activated response was absent; measurement was done at t7.5. Results of measurements with wild type (C, D) stimulated with 6 μM AA are shown for comparison.
Schaloske et al. BMC Cell Biology 2005 6:13 doi:10.1186/1471-2121-6-13