Recordings of Ca2+-fluxes in iplA- and wild type amoebae. [Ca2+]e was measured in cell suspensions with a Ca2+-sensitive electrode. (A) Treatment of amoebae with 5 mM EGTA for 30 min activated capacitative Ca2+-influx (one out of 12/6 determinations in 4/3 independent experiments is shown for iplA- and wild type, respectively). (B) Capacitative influx was blocked by the addition of 1 mM NaN3 (one out of 5/4 determinations in 3/3 independent experiments). Measurements were done at t3.
Schaloske et al. BMC Cell Biology 2005 6:13 doi:10.1186/1471-2121-6-13