Chemotaxis of wild type and mutant amoebae at different experimental conditions. The tracks of individual cells (in red) migrating during chemotactic stimulation (position of the tip of the cAMP-filled capillary: green star) are shown. In H5-buffer (A, B) both cell types migrated in an oriented manner towards the capillary tip, albeit not always in a straight line. After preincubation with 10 mM EGTA for 60 min and in its continued presence during the chemotaxis assay (C, D) the cells remained stationary with random pseudopod extension. Preincubation of amoebae with 10 mM CaCl2 (E, F) did not impair chemotaxis; rather, the cells of both strains migrated towards the capillary tip. Chemotaxis experiments were done at t6.
Schaloske et al. BMC Cell Biology 2005 6:13 doi:10.1186/1471-2121-6-13