[Ca2+]i-recordings in cells preincubated with CaCl2 in order to load stores. (A) The response of wild type upon stimulation with 1 μM cAMP (arrow) at standard conditions, i.e. after preincubation with 1 mM CaCl2 for 10–15 min and stimulated in the presence of 1 mM CaCl2 is shown. Values give mean ± s.e.m. of 7 cells. (B) When iplA- cells were stimulated with 1 μM cAMP at standard conditions (as outlined in (A)), no response was observed. Mean ± s.e.m. of 6 cells is shown. (C) Wild type was preincubated with 1 mM CaCl2 for 4–5 h; after washing cells were incubated in H5-buffer supplemented with 1 μM CaCl2 and challenged with 1 μM cAMP (arrow). Values give mean ± s.e.m. of 16 cells. (D) iplA- incubated for 3 h with 20 mM CaCl2 were washed and subsequently [Ca2+]i-imaging was done in buffer containing 1 mM CaCl2. Arrow indicates the time point when 1 μM cAMP was added. Mean ± s.e.m. of 10 cells is shown.
Schaloske et al. BMC Cell Biology 2005 6:13 doi:10.1186/1471-2121-6-13