Measurement of cAMP activated [Ca2+]i-changes in wild type and mutant amoebae. Cells were stimulated with 1 μM cAMP in the presence or absence of 1 mM external CaCl2. In wild type amoebae a [Ca2+]i-transient was observed in the presence of external Ca2+. The graph shows the average increase (mean ± s.e.m.).
Lusche et al. BMC Cell Biology 2005 6:12 doi:10.1186/1471-2121-6-12