Recombinant SHD pulls down Abi1 and HSPC300. Lysate of Cos7 fibroblasts transfected with HA-Abi1 or HSPC300 was incubated as indicated with GST alone, GST-Scar1 SHD (A), GST-Scar2 SHD (A), or GST-Scar3 SHD (B) on glutathione-s-agarose beads. Beads bound fractions were analysed by SDS-PAGE and immunoblotting with a monoclonal anti-Myc (9E10) antibody. HA-Abi1 and HA-HSPC300 were found in pull-downs with all three Scar Homology Domains, but not with GST alone.
Stovold et al. BMC Cell Biology 2005 6:11 doi:10.1186/1471-2121-6-11