Table 1

Interaction of LPP with Scrib in the yeast two-hybrid system

BAIT

PREY

HIS

LACZ


pGBT9

pACT2-mScrib

-

-

pGBT9-LPPWT

pACT2

-

-

pGBT9-LPPWT

pACT2-mScrib

+

+

pGBT9-LPPS609A

pACT2

-

-

pGBT9-LPPS609A

pACT2-mScrib

+

+

pGBT9-LPPT610A

pACT2

-

-

pGBT9-LPPT610A

pACT2-mScrib

-

-

pGBT9-LPPD611A

pACT2

-

-

pGBT9-LPPD611A

pACT2-mScrib

+

+

pGBT9-LPPL612A

pACT2

-

-

pGBT9-LPPL612A

pACT2-mScrib

-

-


Yeast cells (CG-1945), cotransformed with a bait and a prey as indicated, were selected on medium containing 5 mM 3-AT, lacking Trp, Leu and His. Yeast colonies were tested for the expression of β-galactosidase.

+ indicates strong positive interaction; - indicates no interaction.

Petit et al. BMC Cell Biology 2005 6:1   doi:10.1186/1471-2121-6-1

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