Quantification of the rates of transfection efficiency in the presence of increasing concentrations of LF2000. Upper panel: Flow cytometric analysis showing forward scatter against side scatter of Cho.hIR cells transfected with pGREEN LANTERN using 10 μl LF2000 reagent (ratio of LF2000 to cDNA of 1.1). The separation of intact cells into two subpopulations is indicated by the gates. Percentage values show the relative proportions of the two cell populations compared to the total number of events measured. Lower panel: The proportion of transfected cells in each subpopulation of Cho.hIR cells plotted against the volume of LF2000 reagent used. Data are the average ± S.D. from three experiments which were performed in duplicate for each LF2000 concentration. Filled circles, population A, open circles, population B. *p < 0.01 transfection rate in A> B; **p < 0.001 transfection rate in A > B.
Pramfalk et al. BMC Cell Biology 2004 5:7 doi:10.1186/1471-2121-5-7