Oxidase activity induced by peptide agonists and the effect of PBP 10. Neutrophils were activated by fMLF or WKYMVM and the extracellular release of superoxide anions was recorded by chemiluminescence (expressed in Mcpm). (A) The figure shows the kinetics of the neutrophil response to two concentrations (100 nM and 20 nM) of fMLF or WKYMVM. (B) Dose dependent oxidase activation induced by the two agonists. The peak values were measured and the responses are given as percent of the maximal response. (C) Neutrophils were incubated for 120 minutes in the absence (control) or presence of pertussis toxin (PTX; 500 ng/ml) and the cells were then activated with fMLF (100 nM), WKYMVM (100 nM) or the receptor independent PKC activator PMA (100 nM) (inset). For comparision, the response induced by the two peptides after 90 minutes long incubation time with pertussis toxin is also included. (D) Effect of different concentrations of PBP10 on the neutrophil NADPH-oxidase response induced by fMLF or WYMVM, respectively. Data are expressed as percent of control (without PBP10; mean ± SD of three independent experiments).
Fu et al. BMC Cell Biology 2004 5:50 doi:10.1186/1471-2121-5-50