Table 1

Duration of interphase for daughter cells with and without the spindle midzone or midbody#


midbody/spindle midzone

+
-

spindle midzone (8)
721 ± 66
1444 ± 236**
spindle midzone (5)*
671 ± 28
1724 ± 301**
midbody (9)
610 ± 92
678± 113
control*** (66)
611 ± 16


#Average time in minutes ± SEM

*Average of 5 experiments that showed at least a doubling in the length of interphase for the daughter without the midzone.

**In 3 cases, the daughter cell without spindle midzone failed to enter mitosis during the period of time-lapse recording. For these cells, the length of interphase was assumed to equal the length of observation. Therefore, this average value represents an under-estimate.

***Two unmanipulated cells near the manipulated cell were measured in each experiment as controls.

Murata-Hori et al. BMC Cell Biology 2004 5:49   doi:10.1186/1471-2121-5-49