Table 1

Effect of PE on cell cycle phases.

Treatment
G0/G1 phase
S phase
G2/M phase
% inhibition (S)

vehicle
9269 ± 0.5
319.5 ± 13.5
238.5 ± 23.5
0 %
2 μM PE
9770 ± 9.0
54.0 ± 2.0
125 ± 21.0
83%
5 μM PE
9699 ± 29.0
59.0 ± 4.0
170 ± 11.0
81%
10 μM PE
9761.5 ± 89.5
47.5 ± 13.5
110.5 ± 39.5
85%

Cells were subjected to flow cytometric DNA analysis as described in Methods. Different concentrations of PE inhibited the re-entry of the cell population into S phase of the cell cycle. Data show the number of cells in each phase of the cell cycle. Values are the mean ± S.E. of three independent experiments performed in duplicate on different batches of cells (Total events per cycle is 10,000 cells).

Saeed et al. BMC Cell Biology 2004 5:47   doi:10.1186/1471-2121-5-47