Figure 3.

FRAP of GFP-E-APC and GFP-tagged AJ proteins in early embryonic epithelial cells. Face-on views of live ~6 hours old embryos (stage 11) expressing (a) GFP-E-APC, (b) E-cadherin-GFP, (c) Armadillo-GFP, (d) α-catenin-GFP, with white squares marking sections of cell interfaces that were bleached, and red squares marking unbleached control sections. Pre-bleaching images are shown on the left; subsequent images on the right show recovery of fluorescence within white squares 15, 75 and 300 seconds after bleaching [see additional file 1].