Figure 4.

Simultaneous inhibition of autophagy and the proteasome does not produce additive Rho-mOREG functional responses. CNG cells transiently transfected with Rho-mOREG were assayed for increases in intracellular calcium in response to 10 uM eugenol following treatment with vehicle (control), 10 mM 3-MA, 50 uM MG-132, or 10 mM 3-MA plus 50 uM MG-132 for 4 h. Individual or combined treatment with degradation inhibitors yielded similar levels of Rho-mOREG activity. * p < 0.001 compared to cells treated with vehicle.