Immunogold-based localisation of VHA-a. Ultra-thin cross sections of maize root cells in 1 mm distance to the tip were decorated anti VHA- (A), premmune serum, (B), anti VHA-aN-term-antibody (C,D), respectively. Sections were washed, treated with secondary antibody linked to 15 nm gold particles and visualized in an electron microscope.
Kluge et al. BMC Cell Biology 2004 5:29 doi:10.1186/1471-2121-5-29