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Open Access Research article

Xenopus Cdc14α/β are localized to the nucleolus and centrosome and are required for embryonic cell division

Brett K Kaiser12*, Maxence V Nachury1, Bryan E Gardner13 and Peter K Jackson1

Author Affiliations

1 Departments of Pathology and Microbiology & Immunology and Program in Cancer Biology, Stanford University School of Medicine, Stanford, CA 94305 USA

2 Fred Hutchinson Cancer Research Center, Basic Sciences Division, Seattle, WA 98109, USA

3 University of Pennsylvania School of Nursing, Philadelphia, PA 19104, USA

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BMC Cell Biology 2004, 5:27  doi:10.1186/1471-2121-5-27

Published: 13 July 2004

Abstract

Background

The dual specificity phosphatase Cdc14 has been shown to be a critical regulator of late mitotic events in several eukaryotes, including S. cerevisiae, S. pombe. C. elegans and H. sapiens. However, Cdc14 homologs have clearly evolved to regulate distinct cellular processes and to respond to regulatory signals important for these processes. The human paralogs hCdc14A and B are the only vertebrate Cdc14 homologues studied to date, but their functions are not well understood. Therefore, it is of great interest to examine the function Cdc14 homologs in other vertebrate species.

Results

We identified two open reading frames from Xenopus laevis closely related to human Cdc14A, called XCdc14α and XCdc14β, although no obvious paralog of the hCdc14B was found. To begin a functional characterization of Xcdc14α and XCdc14β, we raised polyclonal antibodies against a conserved region. These antibodies stained both the nucleolus and centrosome in interphase Xenopus tissue culture cells, and the mitotic centrosomes. GFP-tagged version of XCdc14α localized to the nucleulus and GFP-XCdc14β localized to the centrosome, although not exclusively. XCdc14α was also both meiotically and mitotically phosphorylated. Injection of antibodies raised against a conserved region of XCdc14/β into Xenopus embryos at the two-cell stage blocked division of the injected blastomeres, suggesting that activities of XCdc14α/β are required for normal cell division.

Conclusion

These results provide evidence that XCdc14α/β are required for normal cellular division and are regulated by at least two mechanisms, subcellular localization and possibly phosphorylation. Due to the high sequence conservation between Xcdc14α and hCdc14A, it seems likely that both mechanisms will contribute to regulation of Cdc14 homologs in vertebrates.