Figure 5.

Arp3 de-localization consistent with dissociation from the actin network. (A) Time-lapse sequence of a BS-C-1 cell expressing Arp3-GFP shows delocalization of Arp3 within 2 minutes of BDM addition. (B) A linescan of the area marked at 0s (dotted line) plotted over time shows gradual loss of fluorescence with only slight retraction of the leading edge. Washout brings a return of fluorescence in ~1 minute. Bar, 10 um.