TLK1B-KD does not inhibit Aurora B. The reactions in all lanes contained 100 ng of histone H3 (Boehringer Mannheim) and kinase buffer . Lanes 1–4 have 100 ng of Aurora B kinase; Lanes 2–4 have increasing concentration of TLK1-MK, 0.1 μg, 0.3 μg and 1.0 μg respectively. Lanes 5 and 6 are negative controls, lane 5 having only histone H3 while lane 6 has histone H3 and 100 ng of TLK1B-MK (Kinase-dead mutant). The blot was reacted with phospho-histone H3 antiserum.
Sunavala-Dossabhoy et al. BMC Cell Biology 2003 4:16 doi:10.1186/1471-2121-4-16