Expression and purification of GFP-BLM alleles in Sf9 cells. A. Fluorescent micrographs of Sf9 cells infected with recombinant baculoviruses expressing GFP-BLM deletion proteins constructed using methods described previously for the full length normal BLM . The ΔN3 image contains a bi-nucleated cell. B. Silver-stained gels of GFP-BLM deletion proteins isolated in the same manner as normal GFP-BLM . Similar volumes of peak fractions are shown to demonstrate the relative recoveries of the different alleles.
Schawalder et al. BMC Cell Biology 2003 4:15 doi:10.1186/1471-2121-4-15