Table 5

Subsequent stem-cell establishment from the hatched blastocysts derived from IGF-1- or IGFBP-1/IGF-1-supplemented embryo culture.

Culture Medium

No. of hatched blastocysts tested

No. of blastocysts up to three passages (%) to establish ES cell lines

No of wells * (%) of established ES cell lines


kSOM/AA with IGF-1

12

6 (50)

22(45.8) **

kSOM/AA with IGFBP-1/IGF-1

13

8(61.5)

31(59.6) **

kSOM/AA

11

4(36.3)

12(27.3)


The results were pooled from 3 repeats of experiments. * The cellular clump from ICM of each blastocyst was dissociated and implanted into four separate culture wells, which were labeled and traced back after the establishment of ES cell line. ** P < 0.05

Lin et al. BMC Cell Biology 2003 4:14   doi:10.1186/1471-2121-4-14

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