The establishment of ES cell line deriving from the hatching blastocysts. (A) The procedure for establishing ES cell line. (B) When the ICM-derived clump has reached the stage illustrated in figure by day 5–6th after explantation into culture, the ICM-derived component is clearly identified from the trophoblast cell. The cell clump is ready to be disloged and transfered for stem cell culture. (C) Cultured embryonic stem cells following dispersion have been transferred to another culture dish for further growth of the ES cells. (D) Well-established ES cells line, derived from the hatching blastocysts, will aggregate to form a burst-like ES cell colony.
Lin et al. BMC Cell Biology 2003 4:14 doi:10.1186/1471-2121-4-14