fla10 and fla2 average flagellar lengths decrease during incubation at 33°C. Cells were resuspended in pre-warmed buffer at t = 0 h. At least 70 cells were examined per timepoint. wild-type, fla2, fla10. (A) Incubation in HEPES, including contribution of bald and uniflagellate cells.(B) Incubation in HEPES+Ca, including contribution of bald and uniflagellate cells.(C) The same samples as (A), but averages exclude contributions of zero-length flagella.(D) The same samples as (B), but averages exclude contribution of zero-length flagella.
Parker and Quarmby BMC Cell Biology 2003 4:11 doi:10.1186/1471-2121-4-11