Free flagella in Chlamydomonas media are indicative of deflagellation. Determination of free flagella under various conditions. Free flagella observed per 100 cells were counted after 0 and 6 hours at 20°C or 33°C, in either the presence (TAP medium or HEPES+Ca) or absence (HEPES buffer) of calcium, and the difference 6 h – 0 h was calculated. Each data point is the average of at least three experiments on independent cultures.
Parker and Quarmby BMC Cell Biology 2003 4:11 doi:10.1186/1471-2121-4-11