Table 2

TNF-α Enhances the Cytotoxicity of CEES to RAW264.7 Macrophages (24 hours)1

Assay2

Control

LPS

TNF-α

CEES

LPS+CEES

TNF-α +CEES


PI (%)

0.0 ± 0.0a

0.8 ± 0.5a

3.3 ± 1.3a

15.6 ± 4.1b

62.6 ± 1.4c

34.1 ± 7.6b

MTT3

1.68 ± 0.08a

1.61 ± 0.04a

1.55 ± 0.08a

1.37 ± 0.09a

0.32 ± 0.05b

1.08 ± 0.18c


1LPS was used at 25 ng/ml, TNF-α at 50 ng/ml and CEES 500 μM. Values are means ± SD and means (in a given row) not sharing the same superscript letter are significantly different (p < 0.05). 2PI(%) is the percent of propidium iodide fluorescence over that of the control cells. 3The MTT levels are given as 575 nm absorbance units.

Stone et al. BMC Cell Biology 2003 4:1   doi:10.1186/1471-2121-4-1

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