Table 1

IL-1β Enhances the Cytotoxicity of CEES to RAW264.7 Macrophages (24 hours)1

Assay2

Control

LPS

IL-1β

CEES

LPS+CEES

IL1β +CEES


PI(%) 2

0.0 ± 0.0a

10.6 ± 2.1a

10.2 ± 4.1a

64.8 ± 16.9b

120.8 ± 2.3c

111.1 ± 3.9c

MTT3

1.61 ± 0.1b

1.20 ± 0.04a

1.45 ± 0.03b

1.13 ± 0.16a

0.25 ± 0.07c

0.74 ± 0.07d


1LPS was used at 25 ng/ml, IL-1β at 50 ng/ml and CEES 500 μM. Values are mean ± SD and means (in a given row) not sharing the same superscript letter are significantly different (p < 0.05). 2PI(%) is the percent of propidium iodide fluorescence over that of the control cells. 3The MTT levels are given as 575 nm absorbance units.

Stone et al. BMC Cell Biology 2003 4:1   doi:10.1186/1471-2121-4-1

Open Data