The indicated amino acids of C/EBPα were appended to DBD-GFP (see Fig. 1B) and expressed in GHFT1-5 cells. The proportions of green fluorescent cells in G1, S and M phase in nocodazole-treated cells were determined and plotted as the mean +/- standard deviation from three independent experiments. The DBD + 1–154 mutant was repeated a total six times (not shown), for which the statistical significance remained p < 0.01. Statistically significant increases in the proportion of G1 cells, compared to Sham cells, are indicated (One-way ANOVA: **, p < 0.01; *p < 0.05; -, no difference).
Liu et al. BMC Cell Biology 2002 3:6 doi:10.1186/1471-2121-3-6