A, GFP-C/EBP or, B, GFP-C/EBP deleted of amino acids 310 to 358 (ΔLZ) were expressed in GHFT1-5 cells. The ΔLZ construct is depicted in figure 1B. The transfected cells were counter-stained with the blue fluorescent, DNA-binding dye Hoechst 33342 immediately before analysis by fluorescence microscopy. Green fluorescent (left panels) and blue fluorescent (right panels) images were collected from a single cell using filter sets that selectively discriminate GFP and Hoechst 33342 fluorescence (see Materials and Methods). The subnuclear position of GFP-C/EBPα and ΔLZ were compared to the subnuclear position of the peri-centromeric chromatin stained by Hoechst 33342. Representative images are shown.
Liu et al. BMC Cell Biology 2002 3:6 doi:10.1186/1471-2121-3-6