Cells were transfected with the control expression vector (Sham), the transcriptionally active C/EBPα-GFP expression vector or the transcriptionally inactive GFP-C/EBPα expression vector and treated one day later with A, nocodazole or B, mimosine. Cells from the transfections with the C/EBP-GFP and GFP-C/EBP expression vectors were separated into cells with green fluorescence above background levels (C/EBP-GFP and GFP-C/EBP) or at background (No C/EBP). The proportion of cells in the G1, S and G2/M phases were plotted as the mean +/- standard deviation from A, six or B, three independent experiments. Statistically significant differences in the proportion of cells in G1, S or G2/M, relative to the proportions determined for the sham-transfected cells, are indicated (one-way ANOVA: **, p < 0.01; *p < 0.05; -, no difference).
Liu et al. BMC Cell Biology 2002 3:6 doi:10.1186/1471-2121-3-6