Western blot analysis of P0 protein levels with antibodies directed against the V5-epitope. The V5 epitope fused in-frame with P0 reveals expected molecular weights for GFP and GFP fused with P0 proteins. Lane 1: purified recombinant GFP. Lane 2: crude protein extract of sciatic nerve. Lane 3: protein extract of GFP-V5 transfected insect cells. Lane 4: protein extract of wild type P0-GFP-V5 transfected cells. Lane 5: moelcular weight standard.
Ekici et al. BMC Cell Biology 2002 3:29 doi:10.1186/1471-2121-3-29