S2 cells transfected with GFP inserted in the pEXII expression vector. The overall staining of the cells expressing GFP only is clearly visible. Also the nucleus shows green fluorescence in contrast to the cells expressing wild type P0-GFP fusion protein (see fig. 2,5).
Ekici et al. BMC Cell Biology 2002 3:29 doi:10.1186/1471-2121-3-29