Table 3

Transgenic protein and mRNA expression.

Rab27 mutant

line

mRNA expression*

transgenic protein expression


Rab27aT23N

T27aT2

0.20 ± 0.02

not detectable

T27aT15

0.29 ± 0.03

not detectable

T27aT17

0.31 ± 0.01

not detectable

T27aT20

0.29 ± 0.01

not detectable

A27aT6

2.6 ± 0.2

++

A27aT6/18

not determined

++

A27aT13

1.8 ± 0.1

A27aT25/1

0.21 ± 0.03

A27aT25/2

1.7 ± 0.1

+++


Rab27aQ78L

T27Q102

0.32 ± 0.06

A27aQ13

not determined

§

A27aQ24

not determined

§


Rab27aN133I

T27aN14/5

0.61 ± 0.02

not detectable

T27aN48

0.26 ± 0.02

not detectable

A27aN2

0.95 ± 0.04

+

A27aN6

1.27 ± 0.08

+

A27aN14

2.2 ± 0.4

not detectable


Rab27a wt

A27aTwt2

not determined

+++


Rab27bT23N

A27bT40

1.21 ± 0.03

+

A27bT59

0.6 ± 0.1

not detectable


Rab27bN133I

A27bN9

1.64 ± 0.06

++


+++ represents transgenic Rab27 protein level similar to endogenous, ++ or + represent transgenic Rab27 protein level below endogenous, §very high levels of transgenic protein compared to endogenous; * mRNA Rab27 values are expressed as (endogenous + transgenic Rab27a or Rab27b) / Hprt. Endogenous Rab27a/Hprt = 0.22 ± 0.01. Endogenous Rab27b/Hprt = 0.12 ± 0.01.

Ramalho et al. BMC Cell Biology 2002 3:26   doi:10.1186/1471-2121-3-26

Open Data