|
Transgenic protein and mRNA expression. |
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| Rab27 mutant |
line |
mRNA expression* |
transgenic protein expression |
|
|
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| Rab27aT23N |
T27aT2 |
0.20 ± 0.02 |
not detectable |
| T27aT15 |
0.29 ± 0.03 |
not detectable |
|
| T27aT17 |
0.31 ± 0.01 |
not detectable |
|
| T27aT20 |
0.29 ± 0.01 |
not detectable |
|
| A27aT6 |
2.6 ± 0.2 |
++ |
|
| A27aT6/18 |
not determined |
++ |
|
| A27aT13 |
1.8 ± 0.1 |
||
| A27aT25/1 |
0.21 ± 0.03 |
||
| A27aT25/2 |
1.7 ± 0.1 |
+++ |
|
|
|
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| Rab27aQ78L |
T27Q102 |
0.32 ± 0.06 |
|
| A27aQ13 |
not determined |
§ |
|
| A27aQ24 |
not determined |
§ |
|
|
|
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| Rab27aN133I |
T27aN14/5 |
0.61 ± 0.02 |
not detectable |
| T27aN48 |
0.26 ± 0.02 |
not detectable |
|
| A27aN2 |
0.95 ± 0.04 |
+ |
|
| A27aN6 |
1.27 ± 0.08 |
+ |
|
| A27aN14 |
2.2 ± 0.4 |
not detectable |
|
|
|
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| Rab27a wt |
A27aTwt2 |
not determined |
+++ |
|
|
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| Rab27bT23N |
A27bT40 |
1.21 ± 0.03 |
+ |
| A27bT59 |
0.6 ± 0.1 |
not detectable |
|
|
|
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| Rab27bN133I |
A27bN9 |
1.64 ± 0.06 |
++ |
|
+++ represents transgenic Rab27 protein level similar to endogenous, ++ or + represent transgenic Rab27 protein level below endogenous, §very high levels of transgenic protein compared to endogenous; * mRNA Rab27 values are expressed as (endogenous + transgenic Rab27a or Rab27b) / Hprt. Endogenous Rab27a/Hprt = 0.22 ± 0.01. Endogenous Rab27b/Hprt = 0.12 ± 0.01. | |||
Ramalho et al. BMC Cell Biology 2002 3:26 doi:10.1186/1471-2121-3-26 |
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