Half-lives of wild-type, Rab27aT23N, Rab27aQ78L and Rab27aN133I mutant proteins. COS-7 cells transfected with either pCAGGS myc-Rab27aWT (A), pCAGGS myc-Rab27aT23N (B), pCAGGS myc-Rab27aQ78L (C), or co-transfected with pCAGGS myc-Rab27aWT and pCAGGS myc-Rab27aT23N (D) were labelled with [35S]methionine/cysteine for 2 h and subsequently chased in Dulbecco's modified Eagle's medium for the indicated times. Rab27a protein was immunoprecipitated from cell extracts, collected with protein G-Sepharose beads, separated on a 12% SDS-polyacrylamide gel and autoradiographed as described under "Methods". (Panel E) Quantification of radiolabelled samples obtained above.
Ramalho et al. BMC Cell Biology 2002 3:26 doi:10.1186/1471-2121-3-26