Oxidized OxyBURST Green H2HFF BSA distributes in a subset of lysosomes. The Cascade Blue-conjugated dextran was used as a lysosomal marker, and staining of OxyBURST Green H2HFF BSA in PMA-treated cells was performed as described in the Methods section. The area of a cell (indicated by the bracket) was enlarged for comparison, as shown in the inset of each panel. As indicated by the arrows, oxidized dye preferentially accumulates in a subset of lysosomes. Bar, 10 μm.
Chen BMC Cell Biology 2002 3:21 doi:10.1186/1471-2121-3-21